Open AccessRat DNA polymerase β gene can join in excision repair ofEscherichia coli
Author(s) -
Takeo Ohnishi,
Shunsuke Yuba,
Taro Date,
Hiroshi Utsumi,
Akio Matsukage
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.19.5673
Subject(s) - biology , dna polymerase beta , dna polymerase , dna repair , exonuclease , nucleotide excision repair , base excision repair , dna polymerase i , gene , escherichia coli , dna polymerase ii , microbiology and biotechnology , genetics , polymerase chain reaction , reverse transcriptase
Though DNA polymerase I (poll) of Escherichia (E.) coli is understood to play a role in repair synthesis of excision repair, it is still obscure whether DNA polymerase beta (pol beta) plays a similar role in eukaryotic cells. To estimate the role of pol beta in excision repair processes, we inserted the rat pol beta gene into several mutant E. coli defective in a diverse set of enzymatic activities of poll. UV resistance was seen only when the 5'----3' exonuclease (exo) activity of poll molecules remained. Therefore it is suggested that 5'----3' exo activity as well as pol beta activity are essential for repair synthesis of excision repair in eukaryotic cells.