Open AccessDeletion of human JKsegments by site-specific recombination recognizing the conserved nonamer and heptamer sequences
Author(s) -
Tomoyuki Nakatani,
Kazuhiko Horigome,
Noriko Nomura,
Tetsuya Kondo,
Hiroshi Ohtsuka,
Hiroshi Noguchi,
Tasuku Honjo
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.18.5529
Subject(s) - biology , library science , classics , computer science , history
Mapping and partial sequencing of the productive K chain genomic DNA of FK-001 demonstrated a 1.8-kb deletion including the JK2, JK3, JK4, and JK5 segments. This deletion occurred between the heptamer recombination signal sequence of the JK2 segment and the heptamer-like sequence located 1.8 kb downstream of the JK2 segment. The recombination reaction kept the reciprocally joined signal sequences on the chromosome and deleted the intervening DNA segment. The cloned FK-001 K chain gene was expressed efficiently in mouse myeloma cells, demonstrating that the 1.8-kb deleted region conferred no functions for gene expression.