Evidence for a feedback regulated back-up promoter which controls permanent expression of aDictyosteliumgene | Zendy

Markus Maniak | Zendy; Wolfgang Nellen | Zendy

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Evidence for a feedback regulated back-up promoter which controls permanent expression of aDictyosteliumgene

Author(s) -

Markus Maniak,

Wolfgang Nellen

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.18.5375

Subject(s) - biology , dictyostelium , promoter , transcription (linguistics) , gene , response element , regulation of gene expression , gene expression , microbiology and biotechnology , transcription factor , promoter activity , positive feedback , transformation (genetics) , genetics , linguistics , philosophy , electrical engineering , engineering

We have previously described the elements of two overlapping promoters regulating differential expression of the Dictyostelium P8A7 gene. One promoter was constitutively active but stimulated during development (S-promoter), while the other (L-promoter) was found to be induced by a variety of stimuli. This effect was mediated by the induction (i-) element. By a series of antisense transformation experiments we here provide evidence that this element is the target of a feedback control circuit. In wild type cells, feedback regulation activates the L-promoter in unfavourable environmental situations when transcription from the S-promoter is insufficient to generate adequate amounts of protein. A model for the function of the feedback loop under natural and experimental conditions is presented.

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