The URK1 gene ofSaccharomyces cerevisiaeencoding uridine kinase

Uridine kinase (EC 2.7.1.48) catalyses the conversion of uridine ACKNOWLEDGEMENTS into UMP in the pyrimidine salvage pathway of Saccharomyces , , , . , . , T J w •• J O T W * . L I cerevisiae (1, 2). The URK1 gene was doned by complementation l w o u l d ^ d e M o n t 8 y ^ S ' P o t i e r f o r t e c h n i c a l of an uridine kinase deficient strain and is included in a 2.7 kb assistance. DNA segment. A single large ORF following the ATG codon at nucleotide 774 with a stop codon at nucleotide 2276 encodes REFERENCES a polypeptide chain of 501 amino acid residues. Two putative , Grenson,M. (1969) Eur. J. Biochem 11, 249-260. ORF are located on the complementary Strand. ORF1, Of at least 2. Jund.R. (1973) Thesis, University Louis Pasteur, Strasbourg, France. 179 amino acids, initiates upstream from the URK1 gene at nucleotide 537. ORF2, at least 111 amino acids, ends at nucleotide 2367. The screening of the EMBL Data Library did not allow the identification of these ORF.