An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis | Zendy

Masad J. Damha | Zendy; Paul A. Giannaris | Zendy; Steven V. Zabarylo | Zendy

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An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis

Author(s) -

Masad J. Damha,

Paul A. Giannaris,

Steven V. Zabarylo

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.13.3813

Subject(s) - derivatization , biology , oligonucleotide , phase (matter) , chromatography , dna , biochemistry , high performance liquid chromatography , chemistry , organic chemistry

A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required.

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