Nucleotide sequence of hamster glyceraldehyde-3-phosphate dehydrogenase mRNA | Zendy

Sylvie Vincent | Zendy; Philippe Fort | Zendy

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Nucleotide sequence of hamster glyceraldehyde-3-phosphate dehydrogenase mRNA

Author(s) -

Sylvie Vincent,

Philippe Fort

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.10.3054

Subject(s) - biology , nucleic acid sequence , microbiology and biotechnology , nucleotide , hamster , gene , nucleic acid , sequence (biology) , messenger rna , glyceraldehyde 3 phosphate dehydrogenase , genetics , computational biology

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used as an invariant control for gene expression analysis. Only three vertebrate GAPDH cDNAs have been hitherto isolated: human (1), rat (2) and chick (3). In order to get a probe for RNAse-mapping experiments on hamster {Cricetulus griseus) mRNAs, we constructed a cDNA library in pBluescript KSH (Stratagene) from CCL39 cell line (adult lung fibroblastic cells). A full-length cDNA encoding GAPDH was then isolated. DNA sequence was determined on both strands and processed using BISANCE package (CITI2, Pans). The sequence spans 1266 nucleotides, and exhibits a high degree of identity to each of the previously known vertebrates sequences. Northern blot analysis (not shown) revealed the presence of a single mRNA species expressed from hamster GAPDH multigenic family (4).

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