Open AccessCloning and sequence analysis ofgyrAgene ofKlebsiella pneumoniae
Author(s) -
Goberdhan P. Dimri,
H. Das
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.1.151
Subject(s) - biology , dna gyrase , genetics , nucleic acid sequence , klebsiella pneumoniae , open reading frame , gene , homology (biology) , plasmid , pbr322 , coding region , escherichia coli , molecular cloning , sequence analysis , peptide sequence , microbiology and biotechnology
The gene gyrA encoding the DNA gyrase A subunit of Klebsiella pneumoniae has been cloned in the plasmid pBR322. Bases of about 3.5 Kb DNA have been sequenced to locate the gyrA gene. An open reading frame of 2628 nucleotides coding for a 97 KD protein has been identified. Homology to the extent of about 85% was detected at the nucleotide level and about 90% at the amino acid level, when the sequences were compared with that of Escherichia coli gyrA. Some very interesting differences have, however, been found in the promoter region.