Open AccessIdentification of a novel site specific endonuclease produced byMycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines
Author(s) -
N F Halden,
Julie B. Wolf,
Warren J. Leonard
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.9.3491
Subject(s) - biology , microbiology and biotechnology , genetics , library science , computer science
We have discovered a new restriction endonuclease, MfeI, in nuclear extracts from T cells contaminated with Mycoplasma fermentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor alpha chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed.