Identification of a novel site specific endonuclease produced byMycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines | Zendy

N F Halden | Zendy; Julie Wolf | Zendy; Warren J. Leonard | Zendy

Open Access

Identification of a novel site specific endonuclease produced byMycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines

Author(s) -

N F Halden,

Julie Wolf,

Warren J. Leonard

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.9.3491

Subject(s) - biology , microbiology and biotechnology , genetics , library science , computer science

We have discovered a new restriction endonuclease, MfeI, in nuclear extracts from T cells contaminated with Mycoplasma fermentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor alpha chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed.

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