Open AccessUnusual promoter-independent transcription reactions with bacteriophage RNA polymerases
Author(s) -
Guido Krupp
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.8.3023
Subject(s) - biology , transcription (linguistics) , rna , dna , polymerase , primase , primer (cosmetics) , general transcription factor , rna polymerase , genetics , microbiology and biotechnology , transcription bubble , bacteriophage , rna dependent rna polymerase , promoter , reverse transcriptase , gene , gene expression , chemistry , philosophy , linguistics , organic chemistry , escherichia coli
Efficient transcription reactions of DNA-dependent RNA polymerases require the presence of a specific promoter sequence. This report shows that in the absence of their cognate promoter, two bacteriophage RNA polymerases are capable of performing unusual transcription reactions: (i) the DNA template serves also as a primer for RNA synthesis and this leads to hybrid DNA/RNA molecules, (ii) if the DNA template forms a hairpin structure, the linear DNA can be transcribed via the 'rolling circle' mechanism.
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