Open AccessKinetics of exchangeable protons in Z DNA: a UV reaonance Raman study
Author(s) -
Alain Laigle,
L. Chinsky,
PierreYves Turpin,
Béatrice Jollès
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.7.2493
Subject(s) - biology , kinetics , dna , biophysics , microbiology and biotechnology , biochemistry , physics , quantum mechanics
We have studied the hydrogen-deuterium exchange kinetics of the exchangeable protons of the poly(dG-dC).poly(dG-dC) in the Z form of the polymer, using resonance Raman spectroscopy with 257 nm and 284 nm excitation wavelengths. In our experimental conditions (4.5 M NaCl, phosphate buffer pH7, 2 degrees C) the two amino protons and the imino proton of guanine are exchanged with the same exchange half-time of 13 min, whereas the two amino protons of cytosine are exchanged with the same exchange half-time of 51 min.