Kinetics of exchangeable protons in Z DNA: a UV reaonance Raman study | Zendy

Alain Laigle | Zendy; L. Chinsky | Zendy; PierreYves Turpin | Zendy; Béatrice Jollès | Zendy

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Kinetics of exchangeable protons in Z DNA: a UV reaonance Raman study

Author(s) -

Alain Laigle,

L. Chinsky,

PierreYves Turpin,

Béatrice Jollès

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.7.2493

Subject(s) - biology , kinetics , dna , biophysics , microbiology and biotechnology , biochemistry , physics , quantum mechanics

We have studied the hydrogen-deuterium exchange kinetics of the exchangeable protons of the poly(dG-dC).poly(dG-dC) in the Z form of the polymer, using resonance Raman spectroscopy with 257 nm and 284 nm excitation wavelengths. In our experimental conditions (4.5 M NaCl, phosphate buffer pH7, 2 degrees C) the two amino protons and the imino proton of guanine are exchanged with the same exchange half-time of 13 min, whereas the two amino protons of cytosine are exchanged with the same exchange half-time of 51 min.

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