Cloning and DNA sequence analysis of the cDNA for the common α-subunit of the ovine pitultary glycoprotein hormones | Zendy

Paul Bello | Zendy; Peter S. Mountford | Zendy; Malcolm R. Brandon | Zendy; Timothy E. Adams | Zendy

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Cloning and DNA sequence analysis of the cDNA for the common α-subunit of the ovine pitultary glycoprotein hormones

Author(s) -

Paul Bello,

Peter S. Mountford,

Malcolm R. Brandon,

Timothy E. Adams

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.24.10494

Subject(s) - biology , complementary dna , cloning (programming) , library science , cdna library , sequence (biology) , classics , genetics , history , gene , computer science , programming language

The a-subunit is common to the family of heterodimeric glycoprotein hormones FSH, LH, TSH and the placerual chorionic gonadotropin (CG). Clones coding for the pre a-subunit of the pituitary glycoprotein hormones were isolated from a lamb pituitary XgtlO cDNA library [1] using a synthetic oligonucleotide probe based on the published amino acid sequence of the ovine a-subunit [2] and nucleotide sequences for the bovine a-subunit [3,4]. The complete nucleotide sequence as determined by dideoxy sequence analysis [5] and exonuclease HI digestion [6] and the predicted amino acid sequence of one clone, designated o32, is given in Figure 1.

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