Open AccessA negative regulatory element in the promoter of the human α1-antitrypsin gene
Author(s) -
Vincenzo De Simone,
Riccardo Cortese
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.22.9407
Subject(s) - biology , heterologous , promoter , gene , negative regulatory element , regulatory sequence , response element , microbiology and biotechnology , transcription (linguistics) , regulation of gene expression , dna , genetics , gene expression , linguistics , philosophy
A "minimal tissue-specific element" of the human alpha 1-antitrypsin (alpha 1-AT) gene promoter, located in the -137/-37 region, is able to drive hepatoma-specific transcription from the heterologous SV40 promoter. Here we show that, in HeLa cells, this element is inactivated by a negative regulatory mechanism. This negative control can be overcome by deletion or mutation of two specific DNA domains.