Open AccessIdentification of multiplecis-elements andtrans-acting factors involved in the induced expresson of human IL-2 gene
Author(s) -
H. Shibuya,
Tadatsugu Taniguchi
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.22.9173
Subject(s) - biology , trans acting , gene , activator (genetics) , transcription factor , mutant , microbiology and biotechnology , gene expression , regulation of gene expression , regulatory sequence , interleukin 2 , mitogen activated protein kinase , genetics , signal transduction , in vitro
Regulated expression of interleukin-2 (IL-2) gene constitutes an essential part in the clonal proliferation of activated T lymphocytes (T-cells). In order to gain insight on the mechanism(s) of the IL-2 gene induction, deletions were introduced in the human IL-2 gene 5'-flanking region and the mitogen inducibility of each deletion mutant was examined in cultured T cell lines. At least four functional regions were identified, they contain potential binding sites for several transcription factors including one NF-kappa B and two octamer binding factor sites. Whereas each of the functional regions (or elements) is required for the maximal induction of the IL-2 gene by mitogen, one such region was found to be dispensable for activation by the HTLV-1-encoded trans-activator, tax-1. Furthermore, the potent immunosuppressive agent, cyclosporin A was found to inhibit the gene induction by mitogen, but not by tax-1.