z-logo
open-access-imgOpen Access
A group II intron in theNeurosporamitochondrialcolgene: nucleotide sequence and implications for splicing and molecular evolution
Author(s) -
Deborah J. Field,
Adele Sommerfield,
Barry J. Saville,
Richard A. Collins
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.22.9087
Subject(s) - biology , intron , neurospora , genetics , gene , group ii intron , rna splicing , sequence (biology) , group i catalytic intron , nucleic acid sequence , mitochondrial dna , neurospora crassa , rna , mutant
The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J. Mol. Biol. 184: 413-428, 1985). Here we show that coI intron 1 is a typical Group II intron, the only one identified to date in Neurospora. The differential effect of the cyt18-1 mutation suggests that splicing of certain introns could be regulated independently of others by nuclear-encoded proteins. The intron contains a long open reading frame (ORF) resembling that of the Neurospora Mauriceville mitochondrial plasmid. The intron and plasmid ORFs share unusual features of codon usage that suggest both evolved outside of the Neurospora mitochondrial genetic system.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here