Sequence of a cDNA encoding soybean bask 7S globulin
Author(s) -
Hiroyuki Kagawa,
Hisashi Hirano
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.21.8868
Subject(s) - biology , complementary dna , sequence (biology) , cdna library , genetics , computational biology , library science , gene , computer science
Basic 7S globulin (Bg) is a raethionineand cysteine-rich glycoprotein which occurs in soybean seeds (1). The mature Bg is composed of highand low-kDa subunits which are linked by disulfide bonding. Bg is synthesized in the cotyledons during seed development. We isolated a cDNA clone (pBG 108) (sequence below) encoding Bg by using oligonucleotide probes which were constructed on the basis of amino acid sequence data (2). The cDNA contained regions coding for a putative signal peptide (positions 1-72), a high-kDa subunit (73-825) and a low-kDa subunit (826-1281) of Bg.
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