In vitropreferential topoisomerization of bent DNA | Zendy

Micaela Caserta | Zendy; Andrea Amadei | Zendy; Ernesto Di Mauro | Zendy; Giorgio Camilloni | Zendy

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In vitropreferential topoisomerization of bent DNA

Author(s) -

Micaela Caserta,

Andrea Amadei,

Ernesto Di Mauro,

Giorgio Camilloni

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.21.8463

Subject(s) - biology , dna , topoisomerase , hmg box , dna clamp , cleavage (geology) , bent molecular geometry , dna sequencing , microbiology and biotechnology , biochemistry , dna binding protein , gene , polymerase chain reaction , chemistry , reverse transcriptase , paleontology , organic chemistry , fracture (geology) , transcription factor

The steps of the topoisomerization reaction by calf thymus DNA topoisomerase I on a DNA domain containing an intrinsically bent DNA sequence have been analyzed. High preferentiality of binding, cleavage and topoisomerization on the bent segment relative to the rest of the DNA domain was observed. These studies show the importance of the local DNA conformation in the reaction of eukaryotic DNA topoisomerase I and the interest of the use of this enzyme as a tool for the analysis of DNA conformation and DNA dynamics.

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