Open AccessIdentification of two transactlvation domains in the mouse oestrogen receptor
Author(s) -
Jacqueline A. Lees,
Stephen E. Fawell,
Malcolm G. Parker
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.14.5477
Subject(s) - transactivation , biology , receptor , mutant , oestrogen receptor , dna binding protein , binding site , microbiology and biotechnology , plasma protein binding , genetics , transcription factor , gene , cancer , breast cancer
We have identified two discrete transactivation domains within the mouse oestrogen receptor whose relative activities vary according to the target promoter. One domain lies within the N-terminal region and is active in the absence of oestradiol. The second domain is contained within the C-terminal portion of the protein and depends upon oestrogen binding for its activity. The location and oestrogen dependence of this domain has been confirmed using chimaeric receptors containing the Lex A DNA binding domain. Although transactivation by the C-terminal domain is dependent upon ligand binding the analysis of receptor deletion mutants has demonstrated that these two functions are not entirely coincident.