Open AccessMolecular characterization of a gene encoding a photolyase fromStreptomycesgriseus
Author(s) -
Toshihide Kobayashi,
Masashi Takao,
Atsushi Oikawa,
Akira Yasui
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.12.4731
Subject(s) - photolyase , biology , streptomyces griseus , orfs , open reading frame , escherichia coli , dna , peptide sequence , gene , plasmid , streptomyces , saccharomyces cerevisiae , nucleic acid sequence , microbiology and biotechnology , genetics , biochemistry , dna repair , bacteria
By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of Streptomyces griseus. One ORF encodes a polypeptide of 455 amino acids (Mr 50594), which exhibits substantial similarities with the other three photolyases. Photoreactivation-repair deficient E. coli cells could be converted into photoreactivatable ones by introduction of plasmids harboring this ORF, indicating that this is the photolyase gene of S. griseus. The deduced aa sequence of Streptomyces photolyase was most similar to that of E. coli. The putative DNA binding site as well as cofactor binding regions were proposed.