Open AccessIsolation of theXenopusbomolog ofint-1/winglessand expression during neurula stages of early development
Author(s) -
Jaspri. Noordermeer,
Frits Meijlink,
C. Peter Verrijzer,
F.A.M. Rijsewijk,
Olivier Destrée
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.1.11
Subject(s) - biology , xenopus , neurula , complementary dna , microbiology and biotechnology , conserved sequence , peptide sequence , genetics , gene , embryogenesis , gastrulation
We have isolated the Xenopus homolog (Xint-1) of the mouse protooncogene int-1 from a neurula stage 17 cDNA library. The deduced protein sequence of Xint-1 includes 371 amino acids. The Xint-1 protein is more similar to the mammalian int-1 product (69%), than to the Drosophila counterpart of int-1, wingless (50%). Xint-1 shares several characteristics of secreted proteins with the other int-1 homologs: it has a hydrophobic leader, multiple conserved potential N-linked glycosylation sites and is rich in cysteine residues. All 23 cysteines are conserved in the three proteins. Xint-1 is transiently expressed during the neurula stages of early Xenopus development.