Bacillus sphaericusstrain 2297: nucleotide sequence of 41.9 kDa toxin gene | Zendy

J. Roger Hindley | Zendy; Colin Berry | Zendy

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Bacillus sphaericusstrain 2297: nucleotide sequence of 41.9 kDa toxin gene

Author(s) -

J. Roger Hindley,

Colin Berry

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.9.4168

Subject(s) - biology , bacillus sphaericus , bacillaceae , nucleic acid sequence , bacillales , gene , strain (injury) , toxin , microbiology and biotechnology , genetics , sequence (biology) , bacillus (shape) , bacteria , bacillus subtilis , anatomy

We previously reported the nucleotide and deduced amino acid sequences of B. sphaericus 1593 and 2362 41.9kDa larvicldal toxin genes (1 4 2). Both these strains belonged to the same H-flagellar antigen group (H 5a, 5b) and possessed Identical.coding sequences in an open reading frame of 1110 nucleotides. Using restriction fragment probes derived from these sequences we have identified and cloned in pUC12 a 3.5kb Hindlll fragment from B. sphaericus 2297 which contains the entire toxin coding sequence. This strain belongs to a different antigenlc group (H 25) and showed 15 nucleotide changes and a 2 nucleotide insertion when compared with the corresponding sequences from strains 1593 and 2362. Three of these occur upstream of the ORF as does the 2 nucleotide insertion (CT) at position 159-160 (ACTQTTTAA). Within the coding region 12 nucleotide changes were found of which 5 resulted in amino acid changes; V-iF (99), A*S (104), H-

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