Open AccessHeterologous 5' flanking regions do not supportin vitrotemplate activity of aDrosophila melanogastertRNAVal3b gene
Author(s) -
R. A. MacKay,
Dorottya Horváth,
Loverne Duncan,
George B. Spiegelman
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.9.4137
Subject(s) - biology , transfer rna , gene , 5' flanking region , genetics , drosophila melanogaster , transcription (linguistics) , microbiology and biotechnology , gene expression , rna , promoter , linguistics , philosophy
The 5' and 3' structure of a Drosophila tRNA(Val3b) gene was investigated to examine the defect which caused the extremely low in vitro transcription template activity of the gene. Recombinant genes were constructed linking 5' and 3' flanking regions from tRNA genes which were active in vitro templates (tRNA(Val4), tRNA(Arg), tRNA(Ser7)) to the tRNA(Val3b) gene. None of the recombinant genes were effective in vitro templates. The defect in tRNA(Val3b) was demonstrated to reside in the 5' flanking region of the gene and deletion analysis indicated that no specific transcription inhibitor sequence was present 5' to the gene. The data suggest that the effect of 5' flanking sequences on in vitro transcription of the tRNA(Val3b) gene requires a specific relationship between the tRNA gene and the flanking sequence.