Open AccessAdenoslne deaminase gene expression is regulated posttranscriptionally in the nucleus
Author(s) -
Th.M. Berkvens,
F. Schoute,
H. van Ormondt,
P. Meera Khan,
A. J. Van Der Eb
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.8.3255
Subject(s) - biology , microbiology and biotechnology , housekeeping gene , adenosine deaminase , nucleus , gene expression , messenger rna , cell culture , gene , housekeeping , enzyme , biochemistry , genetics
The housekeeping enzyme adenosine deaminase (ADA) shows a large variation in tissue-specific expression ranging from 1 Iu in red blood cells to 880 Iu in thymocytes. We investigated the acute lymphocytic leukemic cell line Molt-4 (660 Iu ADA/g protein) and the promyelocytic cell line HL-60 (38 Iu ADA/g protein) as a model system to determine the levels at which the tissue-specific expression of ADA is regulated. From our results it can be concluded that the almost 20-fold difference in ADA expression between Molt-4 and HL-60 is the result of differences in the post-transcriptional processing and/or stability of ADA pre-mRNA within the nucleus.