The protein factor which binds to the upstream activating sequence ofSaccharomyces cerevisiae ENO1gene | Zendy

Masayuki Machida | Zendy; Hiroshi Uemura | Zendy; Yoshifumi Jigami | Zendy; Hideaki Tanaka | Zendy

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The protein factor which binds to the upstream activating sequence ofSaccharomyces cerevisiae ENO1gene

Author(s) -

Masayuki Machida,

Hiroshi Uemura,

Yoshifumi Jigami,

Hideaki Tanaka

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.4.1407

Subject(s) - biology , saccharomyces cerevisiae , genetics , gene , upstream (networking) , sequence (biology) , fungal protein , computer network , computer science

Using a gel retardation assay it was shown that the 87 bp DNA fragment (UAS87) containing the upstream activating sequence (UAS) of S. cerevisiae EN01 gene and a nuclear extract gave rise to three migration-retarded species specific to UAS87. Heat- or proteinase-treatment of the nuclear extract revealed that these species were protein-DNA complexes. The precise binding region of the protein identified by DNaseI protection analysis was found to include a CCAAACA sequence which forms a dyad-symmetrical structure. The amount of one of the three migration-retarded species significantly increased when cells were grown in medium containing a gluconeogenic carbon source. The introduction of pGCR8, a multicopy plasmid containing GCR1 gene, a regulatory gene controlling the expression of several glycolytic enzymes, showed no effect on the amount of three migration-retarded species.

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