Open AccessTranscription of human 7S K DNAin vitroandin vivois exclusively controlled by an upstream promoter
Author(s) -
Hartmut Kleinert,
Bérnd-Joachim Benecke
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.4.1319
Subject(s) - biology , transcription (linguistics) , upstream activating sequence , rna polymerase ii , microbiology and biotechnology , rna polymerase , gene , rna , rna polymerase iii , rna polymerase ii holoenzyme , dna , polymerase , promoter , coding region , genetics , gene expression , philosophy , linguistics
We have analyzed the transcription of a recently isolated human 7S K RNA gene in vitro and in vivo. In contrast to hitherto characterized class III genes (genes transcribed by RNA polymerase III), the coding sequence of this gene is not required for faithful and efficient transcription by RNA polymerase III. In fact, a procaryotic vector DNA sequence was efficiently transcribed by RNA polymerase III under the control of the 7S K RNA gene upstream sequence in vitro and in vivo. S1-nuclease protection analyses confirmed that the 7S K 5'flanking sequence was sufficient for accurate transcription initiation. These data demonstrate that 7S K DNA represents a novel class III gene, the promoter elements of which are located outside the coding sequence.