Open AccessThe unusually long amino acid acceptor stem ofEscherichia coliselenocysteine tRNA results from abnormal cleavage by RNase P
Author(s) -
Ulrike Burkard,
Dieter Söll
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.24.11617
Subject(s) - biology , selenocysteine , rnase p , transfer rna , escherichia coli , primer extension , microbiology and biotechnology , biochemistry , rna , cleavage (geology) , ribonuclease iii , rnase mrp , rnase h , nucleic acid sequence , gene , genetics , enzyme , paleontology , cysteine , fracture (geology) , rna interference
The nucleotide sequence of the gene encoding the Escherichia coli selenocysteine tRNA (tRNA(SeCys] predicts an unusually long acceptor stem of 8 base pairs (one more than other tRNAs). Here we show by in vivo experiments (Northern blots, primer extension analysis) and by in vitro RNA processing studies that E. coli tRNA(SeCys) does contain this additional basepair, and that its formation results from abnormal cleavage by RNase P.