Open AccessThe regulation of SV40 early gene expression in embryonal carcinoma stem cells-faithful transcriptional regulationin vitro
Author(s) -
Nicholas B. La Thangué,
Peter Rigby
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.24.11417
Subject(s) - biology , enhancer , embryonal carcinoma , cellular differentiation , stem cell , microbiology and biotechnology , retinoic acid , regulation of gene expression , embryonic stem cell , gene , in vitro , endoderm , gene expression , genetics
We have derived from F9 murine embryonal carcinoma stem cells, and from differentiated derivatives induced with retinoic acid and cAMP, whole cell extracts which efficiently and accurately transcribe a variety of supercoiled DNA templates in vitro. These extracts and control elements from viral genomes have been used to study changes in transcriptional activity which accompany differentiation. The SV40 enhancer is inefficiently utilized in stem cells but is activated upon differentiation to parietal endoderm and the in vitro systems mimick this regulation. Mixing experiments demonstrate that the differentiated cell phenotype is dominant, suggesting that stem cells contain limiting amounts of factors required for enhancer activity. Our results may explain the developmental regulation of cellular genes with enhancer motifs in their control sequences.