Open AccessSigma region located between C, and C, genes of human immunoglobulin heavy chain: possible involvement of tRNA-like structure in RNA splicing
Author(s) -
Yasushi Akahori,
Hiroshi Handa,
Kenji Imai,
Masumi Abe,
Kohzoh Kameyama,
Makoto Hibiya,
Hisashi Yasui,
Koji Okamura,
Morihiro Naito,
Hiroshi Matsuoka,
Yoshikazu Kurosawa
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.20.9497
Subject(s) - intron , biology , microbiology and biotechnology , rna splicing , gene , rna polymerase iii , genetics , rna , transfer rna , immunoglobulin heavy chain , base pair , rna polymerase
Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the mu-delta intron, two repeating units were contained. One 442-base-long fragment located JH-mu intron (defined as "sigma mu(sigma mu)") occupied the position in the mu-delta intron. The other 1166-base-long fragment located somewhere in front of S (class switch) region of C gamma gene was also found in the mu-delta intron. We defined the repeats in the mu-delta intron as "SIGMA (sigma)". The polarities of the longer repeats in the genome were opposite between the mu-delta intron and the upstreams of C gamma genes. These inverted copies (defined as sigma gamma 3 and sigma gamma 4), located 6 kb upstream of their respective C gamma's, were apparently transcribed in vitro, via RNA polymerase III and transcripts should have contained tRNA-like structures. Small DNA fragments capable of encoding tRNA-like structures were also found in corresponding regions of mouse Ig C gamma cluster.