Isolation and mapping of a polymorphic DNA sequence (pMCT106) on chromosome 2 [D2S61] | Zendy

Yusuke Nakamura | Zendy; M. Carlson | Zendy; P. O’Connell | Zendy; M. Leppert | Zendy; G.M. Lathrop | Zendy; J.-M. Lalouel | Zendy; R. White | Zendy

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Isolation and mapping of a polymorphic DNA sequence (pMCT106) on chromosome 2 [D2S61]

Author(s) -

Yusuke Nakamura,

M. Carlson,

P. O’Connell,

M. Leppert,

G.M. Lathrop,

J.-M. Lalouel,

R. White

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.19.9359

Subject(s) - biology , genetics , isolation (microbiology) , dna , chromosome , sequence (biology) , dna sequencing , computational biology , gene , bioinformatics

SOURCE/DESCRIPTION; A 1.7 kb Mspl fragment from cosmid MCT106 isolated by an oligonucleotide (GTGTGTGTGTGTGTGTGTGT) (1) was subcloned into the AccI site of pUC18. POLYMORPHISM: PvuII identifies a three allele VNTR polymorphism with bands between 2.0 and 3.5 kb without a constant band. Mspl, TaqI, Rsal, PstI also identify the same polymorphism. FREQUENCY; 32 % hetarozygosity has been observed with PvuII in 114 unrelated Caucasians. NOT POLYMORPHIC FOR: none detected CHROMOSOMAL LOCALIZATION: pMCT106 has been assigned to chromosome 2 by multipoint linkage analysis(2) with loci (ACP, D2S1) known to span this region(3). MENDELIAN INHERITANCE: Co-dominant segregation of the PvuII RFLP was observed in 57 three generation families. PROBE AVAILABILITY: Freely available (contact Y.N.) or will be available from ATCC. OTHER COMMENTS: RFLPs were observed under normal hybridization and washing stringencies.

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