Isolation and mapping of a polymorphic DNA sequence (pEFD145) on chromosome 3 [D3S32] | Zendy

E. Fujimoto | Zendy; Yusuke Nakamura | Zendy; J. Gill | Zendy; P. O’Connell | Zendy; M. Leppert | Zendy; G.M. Lathrop | Zendy; J.-M. Lalouel | Zendy; R. White | Zendy

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Isolation and mapping of a polymorphic DNA sequence (pEFD145) on chromosome 3 [D3S32]

Author(s) -

E. Fujimoto,

Yusuke Nakamura,

J. Gill,

P. O’Connell,

M. Leppert,

G.M. Lathrop,

J.-M. Lalouel,

R. White

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.19.9357

Subject(s) - library science , medical school , white (mutation) , history , medicine , biology , genetics , medical education , computer science , gene

SOURCE/DESCRIPTION: A 4.1 kl) TaqI fragment from cosmid EFD145 was subcloned into the AccI site of pUC18. POLYMORPHISM: Rsal identifies a two allele polymorphism (Rl : 2.4 kb, M2 : 1.4 and 1.0 kb) with a constant band at 0.9 kb. TaqI also identifies a two allele polymorphism (Tl : 5.0 kb, T2 : 4.1 kb) without a constant band, but this observation has not been verified by pedigree studies. FREQUENCY; Estimated from 82 unrelated Caucasians. CHROMOSOMAL LOCALIZATION: pEFD145 has been assigned to chromosome 3 using multipoint linkage analysis(1) with loci (DNF15S2, D3S4) known to span this region(2,3). MENDELIAN INHERITANCE: Co-dominant segregation of the Rsal RFLP was observed in 41 three generation families. PROBE AVAILABILITY: Freely available (contact Y.N.) or will be available from ATCC. OTHER COMMENTS: RFLPs were observed under normal hybridization and washing stringencies.

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