Isotation and mapping of a polymorphic DNA sequence (pEFD134.7) on chromosome 3 [D3S45] | Zendy

Yusuke Nakamura | Zendy; E. Fujimoto | Zendy; Peter O'Connell | Zendy; M. Leppert | Zendy; G.M. Lathrop | Zendy; Jean-Marc Lalouel | Zendy; R. White | Zendy

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Isotation and mapping of a polymorphic DNA sequence (pEFD134.7) on chromosome 3 [D3S45]

Author(s) -

Yusuke Nakamura,

E. Fujimoto,

Peter O'Connell,

M. Leppert,

G.M. Lathrop,

Jean-Marc Lalouel,

R. White

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.19.9355

Subject(s) - biology , genetics , sequence (biology) , dna , chromosome , dna sequencing , microbiology and biotechnology , computational biology , gene

SOURCE/DESCRIPTION; A 4.3 lib Mspl fragment of cosmid EFD134 isolated by a HBV-3 oligonucleotide (1) was subcloned into AccI site of pUC18. POLYMORPHISM; Mspl identifies >10 allelic VNTR polymorphism with bands between 1.5 and 2.7 kb. Rsal and TaqI also identify the same VNTR polymorphism. FREQUENCY: With Mspl, 80 % heterozygosity were observed in 85 unrelated Caucasians. NOT POLYMORPHIC FOR: none detected CHROMOSOMAL LOCALIZATION: pEFD134.7 has been assigned to chromosome 3 by linkage analysis (2) with locus (APOD) known to span this chromosome (3). MENDELIAN INHERITANCE: Co-dominant segregation of the Hspl RFLP was observed in 43 three generation families. PROBE AVAILABILITY: Freely available (contact Y.N.) or will be available from ATCC. OTHER COMMENTS: RFLPs were observed under the normal hybridization and washing condition.

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