Strain polymorphism and tentative mapping of mouse ornithine decarboxylase

SOURCE/DESCRIPTION The plasmld p0D45~contains a 1.6 kb of a omithine decarboxyiase cDNA . POLYMORPHISM Constant Eco RI bands vere found at 3.6, 4.4, 5.5, 6.9, 8.5, 11.5, 13.5 and 19 kb. Variant bands vere found at 4.7 and 6.2 kb. FREQUENCY The 4.7 band is present in BALB/c, not in C57BL/6, DBA/2 and C3Hf, and segregate in CxB recombinant inbred (RI) strains. The 6.2 kb band is present in DBA/2 and C3Hf; these tvo strains can be distinct by the 4.4 band which is present as a doublet in C3Hf. Hind III digestion (not shown) revealed a 12.5 kb band which is present only in DBA/2 DNA. NOT POLYMORPHIC FOR CHROMOSOMAL LOCALIZATION Not reported^ CxB recombinant inbred strains have been used to follow the inheritance of the BALB/c 4.7 kb band. The presence of this band only in CxBG and CxBJ strains delineates the strain distribution pattern BBCBBCB identical for those of VH 3660 (not shown) and of Igh-c gene which has been localized in the distal portion of chromosome 12 (P. D'Eustachio, personal communication). MENDEUAN INHERITANCE Segregation of the 4.7 kb BALB/c band were followed among the CxB RI strains. PROBE AVAILABrrY The probe was obtained from Dr. P. Coffino (University of California, San Francisco). REFERENCES 1) McConlogue L. e t a l . Proc. Na t l . Acad. Sci . USA (1984) 8 1 : 540-544. 2) Brodeur P.M. and Rlble t R. Eur. J . Inmunol. (1984) 14: 922-930. ACKNOWLEDGEMENTS We are grateful to Dr. P. Coffino for the gift of the pOD48 plasmld.