Open AccessThe role of the excision-repair enzymes' in mutation-induction bycic-Pt(NH3)2Cl2
Author(s) -
Jaap Brouwer,
L Vollebregt,
Pieter van de Putte
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.15.7703
Subject(s) - mutagenesis , biology , cisplatin , mutation , nucleotide excision repair , sos response , gene , genetics , enzyme , microbiology and biotechnology , escherichia coli , dna repair , biochemistry , chemotherapy
Mutation induction by cis-Pt(NH3)2Cl2 (cisplatin) has been shown to be absent in E.coli strains carrying a deletion of the uvrB gene (1). This suggested that excision-repair, which is normally thought to be error-free, is involved in mutation induction with cisplatin. Here, the role of the excision repair enzymes UvrA, UvrB and UvrC is investigated using E.coli strains with different repair capacities. It is shown that cisplatin induced mutagenesis is dependent both on UvrA and UvrB but not on UvrC. Of the UvrB enzyme the N-terminal 113 aminoacids are sufficient for mutation induction by cisplatin.