Open AccessMolecular consequences of truncations of the first exon forin vitrosplicing of yeast actin pre-mRNA
Author(s) -
Michael Duchêne,
Angela Löw,
Andrea Schweizer,
Horst Domdey
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.15.7233
Subject(s) - exon , biology , rna splicing , spliceosome , intron , genetics , exon trapping , precursor mrna , exonic splicing enhancer , alternative splicing , messenger rna , yeast , exon shuffling , mature messenger rna , microbiology and biotechnology , gene , rna
A defined minimum length of the first exon is required for the generation of spliced products from a synthetic yeast actin mRNA-precursor in vitro. If the first exon is 1, 2, 3 or 5 nucleotides long, only the first step of the splicing reaction can take place. A transcript starting with the first nucleotide of the intron does not get converted into any of the normally obtained splicing products or intermediates. On the other hand, spliceosome assembly does not depend on the presence of a first exon.