A new approach to the synthesis of a protected 2-aminopurine derivative and its incorporation into oligodeoxynucleotides containing the Eco RI and Bam HI recognition sites | Zendy

Larry W. McLaughlin | Zendy; Terence Leong | Zendy; Fritz Benseler | Zendy; Norbert Piel | Zendy

Open Access

A new approach to the synthesis of a protected 2-aminopurine derivative and its incorporation into oligodeoxynucleotides containing the Eco RI and Bam HI recognition sites

Author(s) -

Larry W. McLaughlin,

Terence Leong,

Fritz Benseler,

Norbert Piel

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.12.5631

Subject(s) - thymine , cytosine , biology , nucleobase , derivative (finance) , nucleoside , helix (gastropod) , guanine , dna , stereochemistry , duplex (building) , purine , biochemistry , nucleotide , chemistry , ecology , snail , gene , financial economics , economics , enzyme

A protected 2-aminopurine nucleoside suitable for incorporation into oligodeoxynucleotides using phosphite triester chemical synthesis procedures has been prepared via oxidation of a purine hydrazino derivative with silver (I) oxide. Five oligodeoxynucleotides containing Eco RI and Bam HI recognition sites have been prepared such that, in the double stranded form, the 2-aminopurine base has either a complementary thymine or cytosine nucleobase. The helix character and thermodynamic parameters for helix formation have been examined.

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