The relative importance ofEscherichia coliexonuclease III and endonuclease IV for the hydrolysis of 3′-phosphoglycolate ends in polydeoxynucleotides | Zendy

Brigitte Siwek | Zendy; S. BricteuxGrégoire | Zendy; Véronique Bailly | Zendy; Walter G. Verly | Zendy

Open Access

The relative importance ofEscherichia coliexonuclease III and endonuclease IV for the hydrolysis of 3′-phosphoglycolate ends in polydeoxynucleotides

Author(s) -

Brigitte Siwek,

S. BricteuxGrégoire,

Véronique Bailly,

Walter G. Verly

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.11.5031

Subject(s) - biology , exonuclease , escherichia coli , exonuclease iii , endonuclease , deoxyribonucleases , hydrolysis , genetics , dna , biochemistry , microbiology and biotechnology , gene , dna polymerase

In vitro, in the presence of Mg++, the 3'-phosphoglycolatase activity of endonuclease IV is about 4-times smaller than that of exonuclease III for the same AP endonuclease activity. It thus seems that endonuclease IV has only a minor role in the repair of strand breaks limited by 3'-phosphoglycolate ends in Escherichia coli even after the amount of enzyme has been increased by induction with O2 -generating agents.

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