Open AccessThe relative importance ofEscherichia coliexonuclease III and endonuclease IV for the hydrolysis of 3′-phosphoglycolate ends in polydeoxynucleotides
Author(s) -
Brigitte Siwek,
S. BricteuxGrégoire,
Véronique Bailly,
Walter G. Verly
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.11.5031
Subject(s) - biology , exonuclease , escherichia coli , exonuclease iii , endonuclease , deoxyribonucleases , hydrolysis , genetics , dna , biochemistry , microbiology and biotechnology , gene , dna polymerase
In vitro, in the presence of Mg++, the 3'-phosphoglycolatase activity of endonuclease IV is about 4-times smaller than that of exonuclease III for the same AP endonuclease activity. It thus seems that endonuclease IV has only a minor role in the repair of strand breaks limited by 3'-phosphoglycolate ends in Escherichia coli even after the amount of enzyme has been increased by induction with O2 -generating agents.