Parameters of field inversion gel electrophoresis for the analysis of pox virus genomes | Zendy

Christopher J. Bostock | Zendy

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Parameters of field inversion gel electrophoresis for the analysis of pox virus genomes

Author(s) -

Christopher J. Bostock

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.10.4239

Subject(s) - biology , genome , pox virus , gel electrophoresis , virus , virology , microbiology and biotechnology , genetics , gene

The effects of variation in the lengths of forward and reverse pulses, voltage gradient, gel concentration and gel temperature on the mobility of DNA molecules in agarose gels during field inversion gel electrophoresis (FIGE) have been determined. A curve, which best fits the empirical data, is presented and allows the choice of pulse conditions and voltage gradient most suitable for the resolution of molecules of chosen size. The use of FIGE in the analysis and direct mapping of large virus genomes is illustrated using vaccinia virus DNA.

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