Expression of thelacZgene from two methanol-regulated promoters inPichia pastoris | Zendy

J Tschopp | Zendy; Paul F. Brust | Zendy; James M. Cregg | Zendy; Cathy A. Stillman | Zendy; T Gingeras | Zendy

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Expression of thelacZgene from two methanol-regulated promoters inPichia pastoris

Author(s) -

J Tschopp,

Paul F. Brust,

James M. Cregg,

Cathy A. Stillman,

T Gingeras

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.9.3859

Subject(s) - pichia pastoris , biology , lac operon , alcohol oxidase , microbiology and biotechnology , promoter , heterologous , gene , pichia , heterologous expression , gene expression , escherichia coli , saccharomyces cerevisiae , genetics , recombinant dna

Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase (AOX) and dihydroxy-acetone synthase (DAS) genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for the expression of the Escherichia coli lacZ gene. These vectors were transformed into P. pastoris host cells and employed in experiments to measure the control mechanisms employed by each promoter in the production of beta-galactosidase fusion products. Results in P. pastoris suggest that the processes used to regulate the expression of these gene fusions involve both repression/derepression and induction mechanisms. Expression of the AOX-lacZ and DAS-lacZ fusions was examined in Saccharomyces cerevisiae as well. Interestingly, beta-galactosidase was expressed in a regulated manner in the heterologous host.

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