Open AccessA new solid-phase synthesis of oligoribonudeotides by the pbosphoro-p-anisidate method using tetrahydrofuranyl protection of 2'-hydroxyl groups
Author(s) -
Shigenori Iwai,
Etsuko Yamada,
Masahiro Asaka,
Yoji Hayase,
Hideo Inoue,
Eiko Ohtsuka
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.9.3761
Subject(s) - biology , nucleotide , tetra , splice , nucleoside , stereochemistry , microbiology and biotechnology , biochemistry , gene , chemistry , paleontology
Six nonaribonucleotides containing the 5'-splice site, one complementary nonamer and an octadecamer containing the 3'-splice site have been synthesized on a polymer support using the phosphoro-p-anisidate method. A 5'-linked 2'-O-tetrahydrofuranyl-N-protected nucleoside 3'-(o-chlorophenyl)phosphoro-p-anisidate was used as the starting nucleotide, and the chain elongated in the 3'-direction by removing the p-anisidate protecting group with isoamyl nitrite under neutral conditions. The octadecamer has been synthesized using dinucleotide blocks and a 3'-terminal trinucleotide.