The nucleotide sequence of a cDNA clone encoding the wheat Emprotein | Zendy

James C. Litts | Zendy; Greggory Colwell | Zendy; Randy L. Chakerian | Zendy; Ralph S. Quatrano | Zendy

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The nucleotide sequence of a cDNA clone encoding the wheat E_mprotein

Author(s) -

James C. Litts,

Greggory Colwell,

Randy L. Chakerian,

Ralph S. Quatrano

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.8.3607

Subject(s) - biology , cyanogen bromide , complementary dna , nucleic acid sequence , peptide sequence , microbiology and biotechnology , clone (java method) , coding region , amino acid , protein sequencing , gene , biochemistry , genetics

The nucleotide sequence of a cDNA clone isolated from developing wheat embryos and encoding the Em protein is reported. The entire coding region for Em and the 3' non-translated flank are contained within this clone. The amino acid sequence deduced for Em is very rich in glycine (18 mol%) as well as both basic and acidic residues. The molecular weight of the protein is ca. 9,900 daltons. The deduced sequence is supported by direct amino acid sequencing of cyanogen bromide cleavage fragments obtained from purified Em protein. Em is shown by Southern blots to be a product of a gene family of approximately ten members.

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