Open AccessRapid hybridization kinetics of DNA attached to submkron latex particles
Author(s) -
Stanley F. Wolf,
Lora L. Haines,
J Fisch,
Jonathan N. Kremsky,
Joseph P. Dougherty,
Kenneth Jacobs
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.7.2911
Subject(s) - biology , dna , nucleic acid thermodynamics , dna–dna hybridization , hybridization probe , microbiology and biotechnology , kinetics , oligonucleotide , rna , conjugate , homogeneous , in situ hybridization , biochemistry , base sequence , gene , messenger rna , mathematical analysis , physics , mathematics , quantum mechanics , thermodynamics
We describe a novel method for attaching any DNA molecule to submicron latex beads and characterize the hybridization kinetic properties of these bead-DNA conjugates. The conjugates hybridize to DNA in solution with rates comparable to homogeneous hybridization reactions, are compatible with common hybridization conditions and are conveniently manipulated. They should thus serve as useful reagents for the fractionation and characterization of DNA and RNA.