Open AccessActivity of aStreptomucestranscriptional terminator inEscherichia coli
Author(s) -
Zixin Deng,
Tobias Kieser,
David A. Hopwood
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.6.2665
Subject(s) - biology , terminator (solar) , escherichia coli , plasmid , nuclease , rna polymerase , streptomyces , dna , microbiology and biotechnology , nucleic acid sequence , genetics , gene , bacteria , ionosphere , physics , astronomy
A 205bp DNA fragment from the Streptomyces multi-copy plasmid pIJ101 has in vivo terminator activity both in Streptomyces lividans and in Escherichia coli. Termination of RNA synthesis, detected by high-resolution S1 nuclease mapping, occurs at precisely the same nucleotides in both organisms. This suggests that the E. coli RNA polymerase recognizes the same sequence elements and chooses the point(s) of termination in the same way as the corresponding S. lividans enzyme.