Open AccessTranscription termination and RNA processin in the 3‘-end spacer of mouse ribosomalo RNA genes
Author(s) -
Takeshi Miwa,
Ryo Kominami,
Hiroshi Yasuda,
Kazuko Sudo,
Masami Muramatsu
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.5.2043
Subject(s) - biology , rna , transcription (linguistics) , rna polymerase i , microbiology and biotechnology , ribosomal rna , small nuclear rna , gene , genetics , rna dependent rna polymerase , spacer dna , dna , intron , internal transcribed spacer , linguistics , philosophy
The 3' termini of ribosomal RNA precursors from mouse FM3A cultured cells are mapped to eight sites within 625 bp downstream from the 3' terminus of 28 S rRNA. Three additional sites are mapped in liver RNA from C3H/He strain mice. Two of them, the sites at 570 bp and 625 bp are assumed to be termination sites in vivo, because they correspond to in vitro termination sites of RNA polymerase I, and 45 S RNAs having these 3' termini decay with kinetics distinct from others. The amount of 45 S RNA having the 3' terminus at other sites is variable among several mouse strains, despite their having the same DNA sequence in these regions. The ability to produce 3' termini in these sites seems to follow Mendel's law of inheritance. Therefore, we postulate that these nine sites are RNA processing sites which are controlled genetically.