Open AccessNucleotide sequence of the pyruvate decarboxylase gene fromZymomonas mobilis
Author(s) -
Alan Neale,
Robert K. Scopes,
Richard E.H. Wettenhall,
Nicholas J. Hoogenraad
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.4.1753
Subject(s) - zymomonas mobilis , pyruvate decarboxylase , biology , biochemistry , nucleic acid sequence , peptide sequence , amino acid , nucleotide , microbiology and biotechnology , gene , enzyme , alcohol dehydrogenase , fermentation , ethanol fuel
Pyruvate decarboxylase (EC 4.1.1.1), the penultimate enzyme in the alcoholic fermentation pathway of Zymomonas mobilis, converts pyruvate to acetaldehyde and carbon dioxide. The complete nucleotide sequence of the structural gene encoding pyruvate decarboxylase from Zymomonas mobilis has been determined. The coding region is 1704 nucleotides long and encodes a polypeptide of 567 amino acids with a calculated subunit mass of 60,790 daltons. The amino acid sequence was confirmed by comparison with the amino acid sequence of a selection of tryptic fragments of the enzyme. The amino acid composition obtained from the nucleotide sequence is in good agreement with that obtained experimentally.