Open AccessCharacterization of the 5′-flanking region for the human fibrinogen β gene
Author(s) -
Philippe Huber,
Jacques Dalmon,
Gilles Courtois,
Monique Laurent,
Zahra Assouline,
G Marguerie
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.4.1615
Subject(s) - biology , 5' flanking region , genetics , gene , homology (biology) , complementary dna , coding region , locus (genetics) , genomic library , restriction map , nucleic acid sequence , gene mapping , microbiology and biotechnology , gene expression , peptide sequence , promoter , chromosome
To identify the possible regulatory sequences in the genetic expression of fibrinogen, a human genomic DNA library raised in lambda EMBL 4 phage was screened using cDNA probes coding for the A alpha, B beta and gamma chains of human fibrinogen. The entire fibrinogen locus was characterized and its organization analysed by means of hybridization and restriction mapping. Among the clones identified, a single recombinant lambda phage contained the beta gene and its 5'- and 3'-flanking regions. A 1.5 kb fragment of the immediate 5'-flanking region was sequenced and S1 mapping experiments revealed three transcription start points. Comparison of this sequence with that previously reported for the same region upstream from the human gamma gene revealed no significant homology which suggests that the potential promoting sequences of these genes are different. In contrast, comparison of the 5'-flanking regions of human and rat beta genes revealed a 142 bp sequence of 80% homology situated 16 bp upstream from the human beta gene. This highly conserved region may well represents a potential candidate for a regulatory sequence of the human beta gene.