Open AccessStructure of theBombyx morirDNA: initiation site for its transcription
Author(s) -
Haruhiko Fujiwara,
Hajime Ishikawa
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.3.1245
Subject(s) - biology , bombyx mori , transcription (linguistics) , primer extension , bombyx , ribosomal rna , nuclease , microbiology and biotechnology , genetics , messenger rna , gene , linguistics , philosophy
The initiation site of rDNA transcription in Bombyx mori was determined to be located at 909bp upstream from the 5'- end of mature 18s rRNA by S1-nuclease mapping and primer extension experiment. An in vitro transcription system, which was constructed using posterior silk glands of Bombyx larvae, initiated the transcription of cloned rDNA at exactly the same site as determined for the in vivo transcription above. The primary transcript seemed to be processed at about 200b downstream of the initiation site both in vivo and in vitro. Sequence analyses of the flanking region of the initiation site revealed that short repetitive sequences are widely distributed throughout the NTS region, and that a highly AT-rich region resides immediately upstream of the initiation region.