Open AccessChicken erythrocyte polynucleosomes which are soluble at physiological ionic strength and contain linker histones are highly enriched hi β-globin gene sequences
Author(s) -
Jenna Ridsdale,
James R. Davie
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.3.1081
Subject(s) - biology , chromatin , histone , histone h2b , globin , dna , biochemistry , nucleosome , gene , microbiology and biotechnology , linker dna , histone h1 , ionic strength , chemistry , aqueous solution
Mature chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength are enriched in beta-globin DNA sequences. Vitellogenin chromatin, which is not expressed in this tissue, is found in aggregation prone, salt insoluble chromatin. There is a direct correlation between the size of soluble fragments and the degree of globin gene enrichment, with the largest fragments being most highly enriched. The highly globin enriched (about 50 fold) polynucleosomes contain significantly elevated levels of acetylated histones H4, H2A.Z, and H2B, and ubiquitinated (prefix "u") histones H2A and H2B (with a significant relative increase of uH2B over uH2A). These polynucleosomes were complexed with histones H1 and H5 but at a lower level than that found in unfractionated chromatin.