Isolation and mapping of a polymorphic DNA sequence pYNZ86.1 on chromosome 3 (D3S30) | Zendy

Yusuke Nakamura | Zendy; Melanie Culver | Zendy; S. Gillilan | Zendy; P. O’Connell | Zendy; M. Leppert | Zendy; G.M. Lathrop | Zendy; J.-M. Lalouel | Zendy; R. White | Zendy

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Isolation and mapping of a polymorphic DNA sequence pYNZ86.1 on chromosome 3 (D3S30)

Author(s) -

Yusuke Nakamura,

Melanie Culver,

S. Gillilan,

P. O’Connell,

M. Leppert,

G.M. Lathrop,

J.-M. Lalouel,

R. White

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.23.10079

Subject(s) - biology , genetics , dna , sequence (biology) , isolation (microbiology) , chromosome , dna sequencing , computational biology , gene , bioinformatics

SOURCE/DESCRIPTION: A 3.3 kb Hindi 11 fragment from cosmid YNZ86 was subcloned into the Hindlll site of pUClB. POLYMORPHISM: Mspl identifies a two allele polymorphism (Ml : 2.0 kb, M2 : 1.9 kb) with constant bands at 2.6 and 1.7 kb. PvuII also identifies a two allele polymorphism (PI : 1.1 kb, P2 : 0.8 kb) with a constant band at 1.5 kb, but this observation has not been verified by pedigree studies. CHROMOSOMAL LOCALIZATION: pYNZ86.1 has been assigned to chromosome 3 by multipoint linkage analysis(l) with loci (DNF15S2, D3S4) known to span this region(2,3>. MENDELIAN INHERITANCE: Co-dominant segregation of the Mspl RFLP was observed in 40 three generation families. OTHER COMMENTS: RFLPs were observed under normal hybridization and wash stringencies.

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