Sequence and identification of the nucleotide binding site for the elongation factor Tu fromThermus thermophilusHB8 | Zendy

Lothar Seidler | Zendy; Marcus E. Peter | Zendy; Franz Meissner | Zendy; Mathias Sprinzl | Zendy

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Sequence and identification of the nucleotide binding site for the elongation factor Tu fromThermus thermophilusHB8

Author(s) -

Lothar Seidler,

Marcus E. Peter,

Franz Meissner,

Mathias Sprinzl

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.22.9263

Subject(s) - thermus thermophilus , biology , ef tu , elongation factor , nucleic acid sequence , gene , thermus , genetics , nucleotide , microbiology and biotechnology , escherichia coli , thermophile , transfer rna , ribosome , bacteria , rna

Two structural genes for the Thermus thermophilus elongation factor Tu (tuf) were identified by cross-hybridization with the tufA gene from E. coli. The sequence of one of these tuf genes, localized on a 6.6 kb Bam HI fragment, was determined and confirmed by partial protein sequencing of an authentic elongation factor Tu from T. thermophilus HB8. Expression of this tuf gene in E. coli minicells provided a low amount of immuno-precipitable thermophilic EF-Tu. Affinity labeling of the T. thermophilus EF-Tu and sequence comparison with homologous proteins from other organisms were used to identify the guanosine-nucleotide binding domain.

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