Open AccessPlant expression signals of theAgrobacteriumT-cyt gene
Author(s) -
Béla Páter,
M. P. Klinkhamer,
P. A. Amesz,
Rolf J. de Kam,
Johan Memelink,
J. H. C. Hoge,
R. A. Schilperoort
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.20.8267
Subject(s) - biology , polyadenylation , tata box , coding region , gene , genetics , start codon , gene expression , microbiology and biotechnology , promoter , agrobacterium , activator (genetics) , regulatory sequence , caat box , messenger rna , transgene
Within the 5' and 3' non-coding regions of the T-cyt gene from the octopine T-DNA of Agrobacterium tumefaciens sequences required for expression of this gene in plant cells were identified by deletion mutagenesis. The results show that 184 bp of the 5' non-coding region and 270 bp of the 3' non-coding region are sufficient for wild-type expression. Within the 5' non-coding region two essential expression signals were identified: (1.) an activator element located between -185 and -129 with respect to the ATG start codon and (2.) one out of two TATA boxes. Deletions of the activator element or the two TATA boxes resulted in nonfunctional genes. Deletion of the upstream TATA box and both putative CAAT boxes did not significantly affect expression. Within the 3' non-coding region, the polyadenylation box most distal to the stop codon was not essential for expression, but sequences more upstream, including a second polyadenylation box were found to be required for wild-type expression.