DNA sequence of theE. coli gyrB gene: application of a new sequencing strategy | Zendy

Toshiro Adachi | Zendy; Michiyo Mizuuchi | Zendy; Elizabeth A. Robinson | Zendy; Ettore Appella | Zendy; M H O'Dea | Zendy; Martin Gellert | Zendy; Kyoshi Mizuuchi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

DNA sequence of theE. coli gyrB gene: application of a new sequencing strategy

Author(s) -

Toshiro Adachi,

Michiyo Mizuuchi,

Elizabeth A. Robinson,

Ettore Appella,

M H O'Dea,

Martin Gellert,

Kyoshi Mizuuchi

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.2.771

Subject(s) - biology , genetics , dna sequencing , gene , dna , sequence (biology) , nucleic acid sequence , base sequence , computational biology , microbiology and biotechnology

We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research