Open AccessDNA sequence of theE. coli gyrB gene: application of a new sequencing strategy
Author(s) -
Toshiro Adachi,
Michiyo Mizuuchi,
Elizabeth A. Robinson,
Ettore Appella,
Mary H. O’Dea,
Martin Gellert,
Kiyoshi Mizuuchi
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.2.771
Subject(s) - biology , genetics , dna sequencing , gene , dna , sequence (biology) , nucleic acid sequence , base sequence , computational biology , microbiology and biotechnology
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.